SKU: 90984444495

3-NT (3-Nitrotyrosine) ELISA Kit

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Description

3-NT (3-Nitrotyrosine) ELISA KitThe 3 NT (3 Nitrotyrosine) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with 3 NT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to 3 NT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate

The 3-NT (3-Nitrotyrosine) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 3-NT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 3-NT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 90984444495

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