Human GSDMD (Gasdermin D) QuickTest ELISA Kit
SKU: 7423298757

Human GSDMD (Gasdermin D) QuickTest ELISA Kit

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Description

Human GSDMD (Gasdermin D) QuickTest ELISA KitThe Human GSDMD (Gasdermin D) QuickTest ELISA Kit measures GSDMD in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into

The Human GSDMD (Gasdermin D) QuickTest ELISA Kit measures GSDMD in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains GSDMD, a capture antibody-GSDMD-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of GSDMD in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 7423298757

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Gregory Howard Cain
Bozeman, US
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Excellent Reference
Format: Kindle
I particularly like Pearls and Pittfalls. These make me mentally prepared before I start a proceedure. Excellent preparation for interventions.
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Reviewed in the United States on October 18, 2015
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pirjee
Bozeman, US
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Very useful for MSK rheumatologists and PMR
Format: Hardcover
Excellent book. A must have for reference.
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Reviewed in the United States on April 12, 2015
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Incision
Houston, US
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Great
Format: Hardcover
Nice addition
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Reviewed in the United States on July 21, 2022
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Anonymous
Carnegie, US
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Five Stars
Format: Hardcover
great book for injections
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Reviewed in the United States on July 24, 2017
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P. B.
Battle Creek, US
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Five Stars
Format: Hardcover
Love this book, extremely useful for some atypical joint injections
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Reviewed in the United States on May 22, 2015

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