Total Estrogen ELISA Kit

Total Estrogen ELISA KitThe Total Estrogens ELISA kit is a competitive ELISA to measure Total Estrogens in serum. Competition occurs between total estrogens (estrone, estradiol, and estriol) present in calibrators, controls, specimen samples and an enzyme labelled antigen (HRP conjugate) for a limited number of anti estrogen antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which

The Total Estrogens ELISA kit is a competitive ELISA to measure Total Estrogens in serum. Competition occurs between total estrogens (estrone, estradiol, and estriol) present in calibrators, controls, specimen samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-estrogen antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of total estrogens present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the color from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of total estrogens in specimen samples and controls can be directly read.